Exosomes are Extracellular Vesicles (EV) that own unique structural features and functions and have gradually become the hot research spot in recent years. The tumor-derived exosomes contain various types of useful biological information, and medical identification of exosomes relied on the specific characterization of membrane surface proteins. In order to rapidly identify non-small cell lung cancer (NSCLC)-derived exosomes based on an aptamer against CD63 protein on exosome membrane, researchers from the Beijing University of Chemical Technology developed a low cost lateral flow aptamer assay (LFAA) test strip using nanogold particles as visualization probes for facile identification of A549 exosomes isolated from human lung carcinoma cells diluted from 6.4 × 109 particles/mL.
A lateral flow aptamer assay strip for facile identification of theranostic exosomes
Yu Q, Zhao Q, Wang S, Zhao S, Zhang S, Yin Y, Dong Y. (2020) Development of a lateral flow aptamer assay strip for facile identification of theranostic exosomes isolated from human lung carcinoma cells. Anal Biochem [Epub ahead of print]. [abstract]