A lateral flow aptamer assay strip for facile identification of theranostic exosomes

exosomes

Exosomes are Extracellular Vesicles (EV) that own unique structural features and functions and have gradually become the hot research spot in recent years. The tumor-derived exosomes contain various types of useful biological information, and medical identification of exosomes relied on the specific characterization of membrane surface proteins. In order to rapidly identify non-small cell lung cancer (NSCLC)-derived exosomes based on an aptamer against CD63 protein on exosome membrane, researchers from the Beijing University of Chemical Technology developed a low cost lateral flow aptamer assay (LFAA) test strip using nanogold particles as visualization probes for facile identification of A549 exosomes isolated from human lung carcinoma cells diluted from 6.4 × 109 particles/mL.

Yu Q, Zhao Q, Wang S, Zhao S, Zhang S, Yin Y, Dong Y. (2020) Development of a lateral flow aptamer assay strip for facile identification of theranostic exosomes isolated from human lung carcinoma cells. Anal Biochem [Epub ahead of print]. [abstract]

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