Aptamer-initiated catalytic hairpin assembly fluorescence assay for universal, sensitive exosome detection

Cancer-cell-derived exosomes are regarded as noninvasive biomarkers for early cancer diagnosis because of their critical roles in intercellular communication and molecular exchange. Researchers from Fudan University have developed a robust aptamer-initiated catalytic hairpin assembly (AICHA) fluorescence assay for universal, sensitive detection of cancer-derived exosomes. The AICHA was verified with the specific detection of MCF-7 cell-derived exosomes with a wide calibration range of 8.4 particles/μL to 8.4 × 105 particles/μL and a low detection limit (LOD) of 0.5 particles/μL. The universality of the AICHA method was verified for PANC-1 cell-derived exosomes, the LOD of which was determined to be 0.1 particles/μL. The performances in serum samples were detected with a recovery rate range of 95.45-106.2%, which demonstrates its significant potential for protein biomarker analysis and cancer diagnosis.

Zhou J, Lin Q, Huang Z, Xiong H, Yang B, Chen H, Kong J. (2022) Aptamer-Initiated Catalytic Hairpin Assembly Fluorescence Assay for Universal, Sensitive Exosome Detection. Anal Chem [Epub ahead of print]. [article]

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