BME Basics: How to extract extracellular vesicles

Cells are constantly communicating, and one way they do this is through extracellular vesicles (EVs), tiny membrane-bound particles that carry proteins and genetic material between cells.

In this episode of BME Basics, MEng student Claire walks through the process of isolating extracellular vesicles from cultured cells. Learn how researchers collect cell culture media, remove unwanted debris through centrifugation, isolate vesicles using exosome precipitation reagents, and visualize the final sample using transmission electron microscopy.

Source – Biomedical Engineering University of Toronto