Comprehensive Exosome Profiling Platforms Regarding Exosomal Contents

Harnessing advanced exosome profiling technology at Creative Biolabs to overcome challenges in the detection, isolation, and analysis of exosomes and their contents.

 The cargo that exosomes contain, including nuclear acids, proteins, lipids, and cytokines, has proven to be potential for cancer diagnostics and the determination of other diseases. However, the small size of exosomes and complex biological environment are technically challenging their molecular detection and analysis, which means exosome isolation is necessary prior to investigating exosomes and expanding their application potentials.

Exosomes exist in plant and animal cells, such as B cells, dendritic cells, and tumor cells, as well as in almost all biological fluids, including plasma, urine, serum, cerebrospinal fluid (CSF), ascites, and saliva. Researchers can isolate exosomes from a variety of cellular debris and interfering components using methods like differential ultracentrifugation, ultrafiltration, precipitation, exosomes capture based on membrane affinity, and isolation based on microfluidic technology.

Scientists at Creative Biolabs, a biotech company deeply engaged in exosome-related services and products for decades, have developed multiple exosome profiling platforms to isolate, purify, quantify, and analyze molecular contents after isolating the required exosomes.

  •  For Exosomal RNA Isolation and Profiling

Exosomes can deliver nucleic acid cargo such as total RNA, mRNA, microRNAs, lncRNAs, and circRNAs for communication between cells. These exosome contents can be extracted using different methods, for example, phenol-based techniques and column-based techniques, after which preliminary characterization is also essential for extracted exosomal RNA. An efficient exosomal RNA profiling platform is developed for the isolation, identification, and properties analysis of RNA contents in exosomes.

  • For Exosomal Protein Isolation and Profiling

Another content that exosomes can recruit and transport based on different cellular origins is cellular proteins, which have been studied as potential biomarkers for cancer, liver diseases, and kidney diseases. Thus, the development of exosomal protein isolation and profiling platform could advance the downstream applications of exosomal proteins including ELISA, protein mass spectrometry, protein biomarker verification, and gel analysis of exosomal proteins.

  • For Exosomal cfDNA Isolation and Profiling

cfDNA, the first detected nucleic acid in the bloodstream, could indicate cell death because cfDNA is believed to be released by dead cells into the blood based on known properties of cfDNA, for instance, its increase in cancer patients and many other disease states. Exosomal cfDNA profiling has become an important step in both fundamental research and clinical applications when it comes to disease diagnosis and novel therapy development.

  • For Exosomal Cytokines Profiling

The ability of exosomes to mediate communications between cells and the microenvironment also depends on cytokines, which are soluble factors secreted through exosomes to regulate immune function and inflammation. Exosomal cytokines profiling can provide multi-parameter analysis of exosomal-related cytokines, possibly identifying exosomes as novel biomarkers in cell-to-cell communication, inflammation, autoimmunity, and infectious disease pathology.

The acquirement and interpretation of profiling data after exosome detection are technically supported by flow cytometry (FC) that can capture the immunomagnetic beads of exosomes, electron microscopy (EM) that can observe the pure and specialized exosomes based on the magnetic separation technology, and optical microscopy (OM) that can observe labeled exosome RNA or membrane components.

About Creative Biolabs

As a company specialized in exosome basic research and application, Creative Biolabs fulfills its mission by providing industry-leading exosomal products and services, striving to help researchers in their projects and advance exosome-related science.

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