Creative Biolabs has developed validated protocols to purify exosomes from both plasma and other body fluids, including ultracentrifugation, microfiltration, size exclusion chromatography, and immunoaffinity capture.
As exosomes can transmit the information of donor cells to recipient cells through the proteins, mRNA, miRNA carried by them, thus to realize the information and material exchange between cells, they have been a hot topic recently as being used as a drug carrier to transport drugs.
“Future research may focus on how to improve the cargo loading of exosomes,” said the senior scientist from Creative Biolabs, “at present, some anti-cancer drugs, including doxorubicin, paclitaxel and genetic drugs, can be effectively loaded into exosomes, which illustrates the potential of exosomes in drug delivery. But the prerequisite for these exosome cargo loading methods is the high-quality purification of exosomes.”
Creative Biolabs believes that the development of efficient, fast, and stable methods that maintain the integrity of the structure and biological function of exosomes is the basis and prerequisite for the current clinical application of exosomes. In view of the advantages and disadvantages of each method, the skillful scientist team can select the appropriate exosome purification method for customers according to the source of the sample and the purpose of the downstream processing.
Creative Biolabs provides the most commonly used ultracentrifugation method, which is known as the “gold standard” for separating and purifying exosome components. It is widely used with its simple operation, no complicated technical support, and relatively low cost.
In the process of exosome purification, microfiltration isolation method can utilize hydrophilized polyvinylidene difluoride membrane to easily purify exosomes from various samples. This technology at Creative Biolabs can reduce co-purification of abundant non-exosomal proteins and increase the purification and integrity of exosomes.
- Immunoaffinity Capture
Creative Biolabs is proficient in the operation of immunoaffinity chromatography. The expert team can provide customized services according to customer needs in selecting specific surface markers of exosome, designing target-specific antibody, and constructing immobilized media for immunoaffinity.
- Size Exclusion Chromatography
In the purification process of exosomes, size exclusion chromatography (SEC) is a good tool for separating the sample components with high yield and high purity. The main advantage of this method is that it does not require a large centrifugal force, thereby ensuring the integrity of the exosomes. The new method based on SEC provides the possibility for large-scale production and clinical application of exosomes.
Further information on exosome-related services and products at Creative Biolabs can be found on https://www.creative-biolabs.com/exosome.