Extracellular vesicles (EVs), such as exosomes and microvesicles, acted as cell-to-cell communication vectors and potential biomarkers for diseases. microRNAs (miRNAs) are the most well studied molecules in EVs, thus a comprehensive investigation of miRNA expression profiles in EVs will be helpful to explore their functions and biomarkers.
Researchers from Huazhong University of Science and Technology curated 462 small RNA sequencing samples of EVs from 17 sources/diseases and constructed the EVmiRNA database to show the miRNA expression profiles. They found >1000 miRNAs expressed in these EVs and detected specific miRNAs for EVs of each source/disease. EVmiRNA provides three functional modules: (i) the miRNA expression profiles and the sample information of EVs from different sources (such as blood, breast milk etc.); (ii) the specifically expressed miRNAs in different EVs that would be helpful for biomarker identification; (iii) the miRNA annotations including the miRNA expression in EVs and TCGA cancer types, miRNA pathway regulations as well as miRNA function and publications. EVmiRNA has a user-friendly web interface with powerful browse and search functions, as well as data downloading. It is the first database focusing on miRNA expression profiles in EVs and will be useful for the research and application community of EV biomarker, miRNA function and liquid biopsy.
A case study of EVmiRNA for B-cell exosome miRNAs
(A) Sample browse for exosomes. (B) The top four highest expressed miRNA in B-cell exosomes. (C) The expression profile of miR-155-5p in each EV. (D) miRNA pathway regulation results figure out that miR-155-5p regulate NFKB signaling pathway and B cell receptor signaling pathway. (E) miR-155 function filtering with ‘B cell
Availability – http://bioinfo.life.hust.edu.cn/EVmiRNA