ExoPRISM – Exosome Precipitation by Ionic Strength Modulation

Extracellular vesicles (EVs) are emerging as crucial materials for precision theragnostic applications. However, current separation methods are time-consuming, costly, and not scalable and deliver limited yields or purity. Researchers at  the Institute for Basic Science, Korea have developed EV precipitation by ionic strength modulation (ExoPRISM), a simple, low-cost, user-friendly, and readily adaptable approach for separating EVs in high yields without compromising their biological functions. Adding an electrolyte solution to blood plasma in small increments generates the sequential precipitation of proteins and EVs, allowing for fractional separation of EVs using low-speed centrifugation. The coprecipitated electrolytes are easily washed away, and the entire EV separation and washing process takes less than an hour. This approach successfully separates EVs from a broad range of volumes and types of biological fluids, including culture medium, urine, plasma, and serum, showing promise as a robust tool for next-generation liquid biopsies and regenerative medicine.

Sunkara V, Park J, Han J, Del Río JS, Cho HJ, Oh IJ, Cho YK. (2023) Exosome Precipitation by Ionic Strength Modulation: ExoPRISM. ACS Appl Mater Interfaces [Epub ahead of print]. [article]

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