Exosome mediated delivery of functional nucleic acid nanoparticles

RNAi-based technologies have shown biomedical potential; however, safe and efficient delivery of RNA remains a barrier for their broader clinical applications. Nucleic acid nanoparticles (NANPs) programmed to self-assemble and organize multiple therapeutic nucleic acids (TNAs) also became attractive candidates for diverse therapeutic options. Various synthetic nanocarriers are used to deliver TNAs and NANPs, but their clinical translation is limited due to immunotoxicity. Exosomes are cell-derived nanovesicles involved in cellular communication. Due to their ability to deliver biomolecules, exosomes are a novel delivery choice.
Researchers from System Biosciences explored the exosome-mediated delivery of NANPs designed to target GFP. The researchers assessed the intracellular uptake, gene silencing efficiency, and immunostimulation of exosomes loaded with NANPs. They also confirmed that interdependent RNA/DNA fibers upon recognition of each other after delivery, can conditionally activate NF-kB decoys and prevent pro-inflammatory cytokines. This study overcomes challenges in TNA delivery and demonstrates future studies in drug delivery systems.

The experimental design of the current work

Exosomes were isolated, packaged with functionalized NANPs and characterized in in vitro and cell culture experiments, demonstrating a novel TNA delivery system.

Nordmeier S, Ke W, Afonin KA, Portnoy V. (2020) Exosome mediated delivery of functional nucleic acid nanoparticles (NANPs). Nanomedicine 30:102285. [article]

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