The research efforts in Associate Professor Samir EL Andaloussi´s group focus on one of the greatest challenges facing modern therapeutics; delivery.
This project will focus on developing a broad and sensitive analytical platform for extracellular vesicle (EV) phenotype and function. Due to the small size of EVs of 100 nm and below, and existing methodological limitations for assessing EV concentrations in biological solutions and quantifying surface protein abundance and other cargo, it has become of utmost importance for all projects aiming to study or use EVs to improve existing methods and develop new ones. It is also a major challenge in the field to establish functional assays to study EV-mediated effects. This optimized platform will be the basis for developing more defined bioengineered extracellular vesicles for treatment of inflammatory diseases.
Most of the research will be conducted utilizing cell lines and animal models. The first part of the work will involve improving existing analytical methods and optimizing new methods for high-resolution analysis of EVs and their phenotypic and functional properties. EV engineering will be employed to generate suitable EV analytical standards and reference materials.
The second part of the work will involve molecular cloning, transduction of cells and subsequent purification of engineered exosomes harbouring therapeutic proteins or RNA, as well as producing standards for analytics and procedures. In doing this, it will be important to phenotype EVs derived from different sources and to follow different engineering strategies, both on bulk mass basis as well as with optical or flow cytometry based single vesicle methods.
The third part involves assessing the functional inter- and intracellular trafficking and biodistribution of these EVs in vitro and in vivo, and to determine their biological activity in cell models as well as in disease animal models.LEARN MORE