ISEV publishes minimal information for studies of extracellular vesicles (MISEV2023): from basic to advanced approaches

Extracellular vesicles (EVs) are tiny, membrane-bound structures released by cells into their surroundings, carrying a payload of proteins, nucleic acids, and other molecules. These vesicles play crucial roles in intercellular communication and are implicated in various physiological and pathological processes.

To ensure that EV research progresses effectively and transparently, the International Society for Extracellular Vesicles (ISEV) introduced the “Minimal Information for Studies of Extracellular Vesicles” (MISEV) guidelines in 2014, with an updated version in 2018. Now, ISEV is proud to announce the latest iteration of these guidelines: MISEV2023.

MISEV2023 aims to provide researchers with an up-to-date framework for studying EVs, covering methods for their production, separation, and characterization across different sources, including cell cultures, body fluids, and solid tissues. By presenting both fundamental principles and cutting-edge techniques, MISEV2023 equips researchers with the tools needed to advance EV research.

One of the notable additions in MISEV2023 is the inclusion of sections on EV release and uptake, shedding light on the mechanisms by which EVs are secreted by cells and taken up by recipient cells. Additionally, the guidelines touch upon in vivo approaches for studying EVs, reflecting the growing interest in understanding EV biology within living organisms.

Position of some EV separation and concentration methods
on a recovery (yield) versus specificity grid

Dashed blue arrows indicate combinations of methods resulting in increased specificity. Specificity can be of different types: Size exclusion chromatography (SEC) separates EVs by size from many (but not all) NVEPs, but all EV types are recovered together, while differential ultracentrifugation (dUC) separates EV subtypes based on their size/weight, but also co-isolates NVEPs at high speeds. Note that many ‘exosome purification’ kits use precipitation (P), thus do not isolate pure exosomes or even EVs but a mixture of EPs, while some use affinity precipitation (AP), which may be more specific to EVs but not exosomes. Those who develop new methods should consider positioning their EV outcomes on such a graph.

MISEV2023 is the result of collaboration among ISEV expert task forces and input from over a thousand researchers worldwide. By synthesizing the collective expertise of the EV research community, these guidelines provide a comprehensive overview of the current state of the field.

By adhering to MISEV2023, researchers can ensure that their studies are conducted with rigor and consistency, facilitating robust scientific discoveries. As EV research continues to evolve, MISEV2023 serves as a valuable resource for navigating the complexities of this rapidly advancing field and unlocking its full potential for biomedical applications.

Welsh JA, Goberdhan DCI, O’Driscoll L, Buzas EI, Blenkiron C, Bussolati B, Cai H, Di Vizio D, Driedonks TAP, Erdbrügger U, Falcon-Perez JM, Fu QL, Hill AF, Lenassi M, Lim SK, Mahoney MG, Mohanty S, Möller A, Nieuwland R, Ochiya T, Sahoo S, Torrecilhas AC, Zheng L, Zijlstra A, Abuelreich S, Bagabas R, Bergese P, Bridges EM, Brucale M, Burger D, Carney RP, Cocucci E, Crescitelli R, Hanser E, Harris AL, Haughey NJ, Hendrix A, Ivanov AR, Jovanovic-Talisman T, Kruh-Garcia NA, Ku’ulei-Lyn Faustino V, Kyburz D, Lässer C, Lennon KM, Lötvall J, Maddox AL, Martens-Uzunova ES, Mizenko RR, Newman LA, Ridolfi A, Rohde E, Rojalin T, Rowland A, Saftics A, Sandau US, Saugstad JA, Shekari F, Swift S, Ter-Ovanesyan D, Tosar JP, Useckaite Z, Valle F, Varga Z, van der Pol E, van Herwijnen MJC, Wauben MHM, Wehman AM, Williams S, Zendrini A, Zimmerman AJ; MISEV Consortium; Théry C, Witwer KW. (2024) Minimal information for studies of extracellular vesicles (MISEV2023): from basic to advanced approaches. J Extracell Vesicles 13(2):e12404. [article]

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