Cells produce two broad classes of extracellular vesicles (EVs), exosomes and microvesicles (MVs). Exosomes are 30–150 nm vesicles derived from multivesicular bodies, while MVs are 200–1,000 nm vesicles that pinch off from plasma membranes. Reliable isolation of EVs is crucial to understand their biochemical and functional properties. Here, Cornell University researchers describe a protocol to isolate and characterize EVs from conditioned medium from mammalian cell lines. This protocol has been optimized for adherent cells but can also be adapted for suspension cells.
Isolation and characterization of extracellular vesicles produced by cell lines
Wang F, Cerione RA, Antonyak MA. (2021) Isolation and characterization of extracellular vesicles produced by cell lines. STAR Protoc 2(1):100295. [abstract]