JOVE publishes a series of exosome technique videos

Exosomes: Vesicles secreted from Multivesicular bodies into the extracellular environment when the multivesicular bodies fuse with the Plasma membrane. Multivesicular bodies are formed from Endosomes when they accumulate vesicles (sometimes referred to as “intraluminal vesicles”) from inward budding of the endosome membrane.


Isolation and Characterization of RNA-Containing Exosomes

1Krefting Research Centre, Department of Internal Medicine, Sahlgrenska Academy, University of Gothenburg

This paper demonstrates methods for the isolation, purification and detection of exosomes, as well as techniques for analysis of their molecular content. These methods are adaptable for exosome isolation from both cell culture media and biological fluids, and can beyond analysis of molecular content also be useful in functional studies.


Detection of Exosomal Biomarker by Electric Field-induced Release and Measurement (EFIRM)

1School of Dentistry, University of California, Los Angeles, 2School of Medicine, Clinical Nutrition, University of California, Los Angeles

Exosomes are microvesicular structures found within biofluids that potentially carry important disease discriminatory biomarkers. Here, a novel method is used to specifically extract exosomes and rapidly test the exosomal cargo for both RNA/protein targets following the disruption of exosomes using non-uniform electric cyclic square waves.


An Innovative Method for Exosome Quantification and Size Measurement

1Research Group Experimental Surgery, Department of Cardiovascular Surgery, Medical Faculty, Heinrich-Heine-University Dusseldorf

A method for isolation of exosomes from whole blood and further analysis by nanoparticle tracking using a semi-automatic instrument is presented in this article. The presented technology provides an extremely sensitive method for visualizing and analyzing particles in liquid suspension.


Quantification and Size-profiling of Extracellular Vesicles Using Tunable Resistive Pulse Sensing

1Department of Neurosurgery, University Medical Center Utrecht, 2Brain Center Rudolf Magnus, University Medical Center Utrecht

Extracellular vesicles play important roles in physiological and pathological processes, including coagulation, immune responses, and cancer or as potential therapeutic agents in drug delivery or regenerative medicine. This protocol presents methods for the quantification and size characterization of isolated and non-isolated extracellular vesicles in various fluids using tunable resistive pulse sensing.

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media

1Department of Pharmacology & Physiology, Drexel University College of Medicine

The presence of stable microRNAs (miRNAs) in exosomes has generated immense interest as a novel mode of intercellular communication, for their potential utility as biomarkers and as a route for therapeutic intervention. Here we demonstrate exosome purification from blood and culture media followed by quantitative PCR to identify miRNAs being transported.
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