The scientific literature contains a wide variety of isolation and characterization procedures for exosomes and other extracellular vesicles. Together with the variable nomenclature, this creates considerable difficulty when comparing results from different independent studies. An additional layer of variability is added by sample handling and pretreatment methodology, where differences arise in terms of the type of blood collection tube, the time between blood draw and generation of plasma or serum, and the measures taken to remove residual cells and cell fragments. This video presents the results of a study comparing handling and pretreatment methods and how they affect the physical characteristics and RNA content of vesicle preparations from a spin column-based purification approach.
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