Measuring concentration of nanoparticles in polydisperse mixtures using interferometric nanoparticle tracking analysis

Quantitative measurement of nanoparticle concentration in liquid suspensions is increasingly crucial across various industries, including medicine and food production. Traditional methods have often struggled with polydisperse samples containing nanoparticles of varying sizes and compositions. Recently, a new technique called interferometric nanoparticle tracking analysis (iNTA) has emerged as a powerful solution to these challenges.

Researchers at the Max Planck Institute for the Science of Light show that iNTA provides a precise method for measuring both the size and refractive index of nanoparticles suspended in liquids. Unlike older methods, iNTA excels at accurately quantifying subpopulations within complex mixtures. It achieves this by counting how many particle trajectories cross a focal plane, eliminating the need for time-consuming calibration samples.

The effectiveness of iNTA has been validated through experiments with both uniform particle samples and mixtures of known concentrations. Researchers have successfully applied iNTA to measure the concentration of SARS-CoV-2 nanoparticles in supernatant samples from infected cells. This application highlights iNTA’s versatility and reliability in biomedical research, where precise measurement of virus particles is essential for understanding infections and developing treatments.

Looking forward, iNTA promises to advance nanoparticle analysis technology, enhancing quality control in pharmaceuticals, diagnostics, and food safety. Its ability to handle complex nanoparticle mixtures with accuracy positions it as a pivotal tool for driving scientific progress and innovation across diverse fields.

Kashkanova AD, Albrecht D, Küppers M, Blessing M, Sandoghdar V. (2024) Measuring Concentration of Nanoparticles in Polydisperse Mixtures Using Interferometric Nanoparticle Tracking Analysis. ACS Nano [Epub ahead of print]. [article]

Leave a Reply

Your email address will not be published. Required fields are marked *