Myocardial infarction is the leading cause of morbidity and mortality worldwide. Recent advances in cardiac regenerative therapy have allowed for novel modalities in replenishing the damaged myocardium. However, poor long-term engraftment and survival of transplanted cells have largely precluded effective cell replacement. As an alternative to direct cell replacement, the release of paracrine protective factors may be a more plausible effector for cardioprotection which may partially be mediated through secretion of microvesicles, or exosomes, that contribute to cell-cell communication. Stanford University School of Medicine researchers describe the isolation of exosomes from induced pluripotent stem cells-derived cardiomyocytes for subsequent microRNA profiling for a better understanding of the biological cargo contained within exosomes.
Electropherogram of exosomal small RNAs isolated from iPSC-CMs conditioned medium measured by Bioanalyzer 2100 with the Small RNA assay
The electropherogram is plotted as fluorescence intensity (Y-axis) versus size (X-axis). Two distinct regions are defined with the Small RNA assay including small RNA region from 0 to 150 nt (delineated by dashed green lines) and the microRNA region from 10 to 40 nt (delineated by dotted gray lines). Nearly 40% of miRNAs were detected for this particular sample. The data can also be displayed as a densitometry plot as shown on the right