MoBiTec to Offer ExoTEST™ and Exo-FACS: Exosome Quantification Kits

Exosome research is a novel field of biomedical research with implications in vaccines and cancer. The use of exosomes for assessment of functional state of parent cells in research is currently hampered by the lack of reliable and affordable tools.

MoBiTec together with HansaBioMed present ExoTEST™, an innovative technology that allows purification, quantification, and characterization of circulating exosomes specific to a cell type or tissue, or to a condition (health vs. disease). Together with ExoTEST™ HansaBioMed team has developed a new kit for FACS (Fluorescence Activated Cell Sorting) analysis of exosome markers, Exo-FACS, which allows exosome FACS profiling without wasting time in exosome isolation by ultracentrifugation.

ExoTEST™ is a patented double sandwich ELISA assay for quantitative and qualitative analysis of exosomes. In particular, ExoTEST™ is a successful platform for exosome quantification and characterization from small amount of human biological fluids or cell media and it may be exploited to identify exosomes released by cancer cells in the plasma and urine of tumor patients in various disease conditions. ExoTEST enables reliable and precise quantitative measurement and comparison among samples and individual experiments and provides increased sensitivity in detection of exosomal markers with respect to other analytical methods (like FACS, WB).

Applications
• Exosome capture and quantification from human biofluids and cell culture media
• Exosome comprehensive profiling
• Pre-clinical research on non-invasive biomarkers for detection and monitoring of a number of pathological conditions
(inflammation, cancer, neurodegeneration, etc.)

Exo-FACS allows exosome isolation from biofluids or cell culture media and FACS analysis of exosome markers. The kit consists of EXO-Prep reagent for exosome isolation, 4 µm beads used for the overall capture of pre-isolated exosomes, lyophilized exosomes from cell culture supernatants or human biological fluids, as positive control. The characterization of exosomal proteins (membrane expressed or internal) is subsequently performed using appropriate detection antibodies against exosome associated antigens.

Applications
• Exosome isolation and exosome marker characterization via FACS
• Exosome comprehensive profiling

Source – Open PR