Nanoparticles to help evaluate the effectiveness of radiation therapy for cervical cancer

Russian scientists have developed a new method for evaluating the effectiveness of radiation therapy for cervical cancer patients. The study shows that using scanning electron microscopy to analyze changes in the morphology of erythrocytes under the influence of ionizing radiation during RT not only allows the detection of nanosized extracellular vesicles on the surface of erythrocytes but also can be used as a criterion for assessing the recovery period.

Current estimates indicate that every year over 16 thousand women are diagnosed with cervical cancer in Russia. Cervical cancer ranks as the 5th most frequent cancer among the country’s women. The increase in relapses or further development of the disease after a course of therapy, including a course of radiation therapy (RT), poses a challenge for researchers to develop diagnostics for the effectiveness of therapy. A team of Russian scientists has developed a new method for evaluating the effectiveness of radiation therapy for cervical cancer by analyzing a set of blood parameters of CC patients using scanning electron microscopy (SEM).

Scientists from NUST MISIS, M.K. Ammosov North-Eastern Federal University, Yakut Scientific Center of Complex Medical Problems, Republican Oncology Dispensary, Lomonosov Moscow State University analyzed venous blood samples from patients diagnosed with CC. Changes were found in the morphology of erythrocytes of CC patients, as well as in the number and size of nanoparticles found on their surface at different stages of RT.

According to the scientists, there are two types of nanoparticles (nanoviruses and extracellular vesicles) that appear on the cell surface during the course of radiation therapy. The researchers found that the amount of nanoviruses detected in some samples decreased during RT, while extracellular vesicles were detected precisely during RT and their number both increased and decreased during therapy.

NP-objects on the RBC surface before (A) and during (B) RT in a blood sample from a patient with CC (magnification of 40,000x, an accelerating voltage of 1 kV).

Further observation of patients with CC after RT showed that an increase in the number of vesicles in the blood may be associated with a negative prognosis related to a relapse of the disease, i.e. about the low efficiency of RT. In addition, scientists suggest that vesicles localized on the cell surface can cause further development of the disease by transferring it to other organs and tissues.

“It is known that erythrocytes carry gases (oxygen, nitric oxide, etc.), and also have receptors for a number of hormones and mediators (insulin-like growth factor, ATP) necessary for the regulation of the body’s vital functions. We discovered another mechanism: along with erythrocytes, nanosized vesicles and other nanoparticles that appear on the surface of erythrocytes as a result of the pathology can also spread throughout the body. For example, the presence of such nanosized particles in human blood may indicate the development of cancer. By analyzing changes in the morphology and composition of nanoscale vesicles, one can monitor the effectiveness of radiation therapy. The correlation between the number of viruses detected by SEM and vesicles on the cell surface may also be the basis for the formation of new diagnostic method,” noted Prof. Grigory Maksimov, NUST MISIS.

Further examination of the blood of patients during RT will make it possible to reveal the quantitative ratio of these nanoparticles before and after each stage of RT to determine the criterion (critical ratio) of the effectiveness of therapy. Thus, the complex technique for examining the blood of CC patients developed by the Russian scientists can become the basis for developing a methodology for determining the effectiveness of RT, as well as form the basis for further studies of the role of viruses, vesicles, and erythrocytes in the development of CC.

SourceEurekalert

Mamaeva SN, Kononova IV, Alekseev VA, Nikolaev NA, Pavlov AN, Semenova MN, Maksimov GV, Ammosov MK. (2021) Determination of Blood Parameters using Scanning Electron Microscopy as a Prototype Model for Evaluating the Effectiveness of Radiation Therapy for Cervical Cancer. Int J of Biomed 11(1);32-38. [article]

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