NanoString’s Hyb & Seq™ Enables Liquid Biopsy by the Direct Capture and Sequencing of Cell-Free DNA

NanoString Technologies, Inc, a provider of life science tools for translational research and molecular diagnostic products, today announced that the company has demonstrated the capability of its Hyb & Seq technology to perform liquid biopsy measurement.  The proof-of-principle work was presented at the Advances in Genome Biology and Technology (AGBT) Precision Health meeting held in Scottsdale, Arizona, September 14-16, 2017.

The sequencing of circulating cell-free DNA (cfDNA) from blood is a promising non-invasive tool for clinical oncology. Hyb & Seq offers numerous advantages over current NGS methods by leveraging a targeted single molecule sequencing workflow to directly capture and analyze cfDNA without amplification or library construction. The well-characterized extraction process ensures high yield of cfDNA with minimal contamination from cellular genomic DNA. Hyb & Seq technology enables accurate sequencing without the need for complex and time consuming library preparation, enzymes or amplification, allowing the efficient capture of cfDNA to be completed in approximately 60 minutes.

“Our work demonstrates that Hyb & Seq is capable of performing precise measurement of cell-free DNA, which could enable rapid and accurate liquid biopsies,” said Joe Beechem, senior vice president of R&D of NanoString Technologies. “Having already established the rapid, simple sequencing of FFPE samples, we are now well-positioned to address the two most important sample types in oncology, underscoring the tremendous commercial potential for Hyb & Seq.”

The company presented two posters at the AGBT Precision Health meeting, the first of which is titled “Eliminating sample and NGS library preparation bias and bottlenecks for liquid biopsy applications with Hyb & Seq technology” describing the workflow for isolating cfDNA from plasma, and the performance of capturing and detecting it with Hyb & Seq.  The Hyb & Seq liquid biopsy isolation protocol provided cfDNA of a quantity and quality comparable to commercially available kits.  Reference cfDNA extracted from synthetic plasma was used to assess the reproducibility of cfDNA sample preparation and sequence detection using a prototype Hyb & Seq system.  When processing a 100+ target panel from synthetic reference plasma, the Hyb & Seq liquid biopsy process delivered  highly reproducible (R2 = 0.93) detection.

A second poster titled “Direct, simultaneous sequencing of DNA and RNA in less than 60 minutes from FFPE samples using Hyb & Seq Technology” demonstrated recent improvements in performance and workflow when processing formalin-fixed, paraffin-embedded tissue samples.  Direct RNA sequencing by Hyb & Seq showed strong correlation (R2 = 0.99) with results from the nCounter® Analysis System.  In addition, a streamlined sample preparation workflow was demonstrated, reducing hands-on time from 15 minutes to 10 minutes.

About Hyb & Seq Technology
Hyb & Seq is a novel single molecule sequencing technology being developed by NanoString. The platform enables a workflow that is simpler and faster than current sequencing methods because it does not require library preparation, enzymes or amplification. Hyb & Seq technology’s simplicity, flexibility, and accuracy offer the potential for an ideal sample-to-answer solution for clinical sequencing. In proof-of-concept experiments, the Hyb & Seq chemistry has demonstrated:

  • A low intrinsic error rate and the ability to provide high consensus accuracy at low coverage by non-destructively sequencing the same native molecule multiple times
  • Simultaneous capture and sequencing of DNA and RNA molecules in a single experiment
  • Both short and long read capabilities, with demonstrated read lengths up to 33kb and no theoretical upper limit
  • Total processing time from FFPE sample to start of sequencing of under 60 minutes, and hands-on time of less than 15 minutes

Hyb & Seq technology is currently for research use only and is not for use in diagnostic procedures.

Source – Globe Newswire

Leave a Reply

Your email address will not be published. Required fields are marked *