Novel therapy harnesses stem cell-derived exosomes for diabetic wound healing

Researchers unveiled a novel therapy for diabetic wound healing. This research highlights the use of exosomal miR-4645-5p from hypoxic bone marrow mesenchymal stem cells (BMSCs) to significantly enhance wound healing by promoting keratinocyte autophagy.

Diabetic wounds, often challenging to treat and prone to complications, can severely impact patients’ quality of life. Traditional treatments have struggled with issues like low survival rates of transplanted cells and potential for immune rejection. This research introduces a groundbreaking approach using stem cells’ regenerative capabilities.

In a recent study published in the journal Burns & Trauma, researchers at Nanchang University have pioneered a novel approach to heal diabetic wounds faster and more effectively than ever before. Their research centers on the use of special particles called exosomes, which are derived from stem cells grown under low oxygen conditions, known as hypoxic conditions. These exosomes contain a potent molecule, miR-4645-5p, that significantly boosts the healing process.

Schematic representation of the therapeutic effect of hyBMSC-Exos on diabetic wounds

Schematic representation of the therapeutic effect of hyBMSC-Exos on diabetic wounds. The schematic graph shows that hyBMSC-Exos promotes wound healing by promoting keratinocyte autophagy. Specifically, hyBMSC-Exo-mediated transfer of miR-4645-5p inactivates MAPKAPK2-induced AKT-mTORC1 signaling in keratinocytes, leading to keratinocyte autophagy, proliferation and migration. BMSC bone marrow mesenchymal stem cell, hyBMSC-Exo hypoxic BMSC-sourced exosome, AKT AKT kinase group [AKT1 (AKT serine/threonine kinase 1), AKT2 and AKT3], mTORC1 mechanistic target of rapamycin kinase complex 1, RPS6KB1 ribosomal protein S6 kinase B1

The schematic graph shows that hyBMSC-Exos promotes wound healing by promoting keratinocyte autophagy. Specifically, hyBMSC-Exo-mediated transfer of miR-4645-5p inactivates MAPKAPK2-induced AKT-mTORC1 signaling in keratinocytes, leading to keratinocyte autophagy, proliferation and migration. BMSC bone marrow mesenchymal stem cell, hyBMSC-Exo hypoxic BMSC-sourced exosome, AKT AKT kinase group [AKT1 (AKT serine/threonine kinase 1), AKT2 and AKT3], mTORC1 mechanistic target of rapamycin kinase complex 1, RPS6KB1 ribosomal protein S6 kinase B1

This study delves into the use of exosomes from hypoxic bone marrow mesenchymal stem cells (BMSCs) as a novel treatment for diabetic wounds, which are notoriously difficult to heal and prone to infections. Focusing on the microRNA miR-4645-5p, found within these exosomes, the research uncovers its critical role in enhancing wound healing by targeting the MAPKAPK2 pathway, thereby regulating the AKT-mTORC1 signaling cascade. This inhibition boosts autophagy in keratinocytes—key players in wound repair—by promoting cell health, proliferation, and migration. Demonstrating that exosomes enriched with miR-4645-5p from hypoxic BMSCs can significantly speed up the healing of diabetic wounds, the study paves the way for new regenerative medicine strategies that manipulate cellular environments to enhance autophagy, offering a promising avenue to improve outcomes in diabetic wound care.

Our findings offer a new horizon in diabetic wound care. By harnessing the power of stem cell-derived exosomes, particularly under hypoxic conditions, we’ve seen a remarkable improvement in wound healing processes, opening doors to potentially life-saving treatments.” Dr. Yan Shi, lead researcher

This research not only sheds light on the mechanisms behind stem cell-mediated wound healing but also opens up new avenues for developing treatments for diabetic wounds and possibly other conditions. The ability to harness and modulate the healing properties of stem cells through their exosomes could lead to more effective, targeted therapies, reducing the burden of diabetic wounds on patients worldwide.

Source – TranSpread

Shi Y, Wang S, Liu D, Wang Z, Zhu Y, Li J, Xu K, Li F, Wen H, Yang R. (2024) Exosomal miR-4645-5p from hypoxic bone marrow mesenchymal stem cells facilitates diabetic wound healing by restoring keratinocyte autophagy. Burns Trauma 12:tkad058. [article]

Leave a Reply

Your email address will not be published. Required fields are marked *

*