On‐chip biogenesis of circulating natural killer cell‐derived exosomes

As the recognition between natural killer (NK) cells and cancer cells does not require antigen presentation, NK cells are being actively studied for use in adoptive cell therapies in the rapidly evolving armamentarium of cancer immunotherapy. In addition to utilizing NK cells, recent studies have shown that exosomes derived from NK cells also exhibit antitumor properties. Furthermore, these NK cell‐derived exosomes exhibit higher stability, greater modification potentials and less immunogenicity compared to NK cells. Therefore, technologies that allow highly sensitive and specific isolation of NK cells and NK cell‐derived exosomes can enable personalized NK‐mediated cancer therapeutics in the future.

Researchers from the University of Michigan propose a novel microfluidic system to collect patient‐specific NK cells and on‐chip biogenesis of NK‐exosomes. In a small cohort of non‐small cell lung cancer (NSCLC) patients, both NK cells and circulating tumor cells (CTCs) were isolated, and the researchers found NSCLC patients have high numbers of NK and NK‐exosomes compared with healthy donors, and these concentrations show a trend of positive and negative correlations with bloodborne CTC numbers, respectively. They further demonstrate that the NK‐exosomes harvested from NK‐graphene oxide chip exhibit cytotoxic effect on CTCs. This versatile system is expected to be used for patient‐specific NK‐based immunotherapies along with CTCs for potential prognostic/diagnostic applications.

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Schematic diagram of the microfluidic technology approach for on‐chip natural killer (NK) cell isolation, in situ NK cell‐derived exosome biogenesis, and recovery for potential therapeutic use of NK exosomes.

Kang YT, Niu Z, Hadlock T, Purcell E, Lo TW, Zeinali M, Owen S, Keshamouni VG, Reddy R, Ramnath N, Nagrath S. (2021) On‐Chip Biogenesis of Circulating NK Cell‐Derived Exosomes in Non‐Small Cell Lung Cancer Exhibits Antitumoral Activity. Adv Sci [Epub ahead of print]. [article]

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