Extracellular vesicles (EVs) are critical mediators of intercellular communication, capable of regulating the transcriptional landscape of target cells through horizontal transmission of biological information, such as proteins, lipids, and RNA species. Given the increasing interest of EV biology in many research disciplines, especially in the area of cancer biology and translational research, this current Special Issue (released in two separate editions in 2019) attempts to provide readers with an up‐to‐date overview on some of the current developments in this expanding field. This issue focuses on the contribution of proteomics for the cataloguing, characterization, and quantification of protein constituents across EV subtypes and more importantly, their biogenesis, trafficking, uptake, and biological function. Such advances in proteomics and developments in technology and the way EVs are defined can now be directed towards further investigating EV biology, especially to sub‐organelle studies and promises to revolutionize our understanding of these extracellular moieties.
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