Protocol for cross-platform characterization of human and murine extracellular vesicles and particles

Characterization of isolated extracellular vesicles and particles (EVPs) is crucial for determining functions and biomarker potential. Researchers at Weill Cornell Medicine present a protocol to analyze size, number, morphology, and EVP protein cargo and to validate EVP proteins in both humans and mice. The researchers describe steps for nanoparticle tracking analysis, transmission electron microscopy, single-EVP immunodetection, EVP proteomic mass spectrometry and bioinformatic analysis, and EVP protein validation by ExoELISA and western blot analysis. This allows for EVP cross-validation across different platforms.

Bojmar L, Kim HS, Sugiura K, Heissel S, Lucotti S, Cioffi M, Johnson KE, Cohen-Gould L, Zhang H, Molina H, Matei IR, Lyden D, Hoshino A. (2023) Protocol for cross-platform characterization of human and murine extracellular vesicles and particles. STAR Protoc 5(1):102754. [article]

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