Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media

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The presence of stable microRNAs (miRNAs) in exosomes has generated immense interest as a novel mode of intercellular communication, for their potential utility as biomarkers and as a route for therapeutic intervention. Here researchers from  Drexel University College of Medicine demonstrate exosome purification from blood and culture media followed by quantitative PCR to identify miRNAs being transported.

[framed_box]McDonald, M. K., Capasso, K. E., Ajit, S. K. Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media. J. Vis. Exp. (76), e50294, doi:10.3791/50294 (2013).

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