RNA-Seq of Exosomes

Exosomes represent a novel mode of cell communication, involved in material transport, signal transmission, and gene expression. Studies have found that ncRNAs in exosomes such as miRNAs can mediate communication between diverse cell types, including immune cells. Cellular exchanges of RNA via exosomes contribute to adaptive and innate immune responses, influencing biological reproductive developmental processes and disease onset, including cancer, neurodegenerative diseases, and viral infections. Sequencing technology has advanced successfully to become one of the most used routine tools for life science research. Transcriptome profiling that uses sequencing technologies can supply valuable information. Here is a standardized workflow and protocol for the isolation of exosomes and downstream analysis of exosomal RNA.