Setting a Cornerstone for Platform Purification of Exosomes

From BioProcess International

Exosomes are a subject of rapidly growing therapeutic interest in the biopharmaceutical industry for two principal reasons. The first reason is that they are the primary communicators of instructions from source cells to target cells. Exosome surface features define their destination. They recognize complementary features on target cells, dock with them, and deliver their programmed instructions in the form of microRNA. The second reason is that exosomes are immunologically silent. As normal human cell products, and by contrast with gene therapy vectors such as virus particles, exosomes bypass the issue of triggering an immune response that might interfere with therapy.

Chromatin structure and nucleosome array distribution in mammalian cell culture harvest (cch); agarose electrophoresis of Chinese hamster ovary cch is shown on the right. Each band in the cch corresponds to a nucleosomal array. The numerals at far right indicate the number of nucleosomes in each array. Note that the number of base pairs per array increases by increments of about 150, consistent with the known number of base pairs per nucleosome.

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