Researchers at Chongqing Medical University have developed a label-free and real-time surface plasmon resonance imaging biosensor based on the hydrogel-AuNP supramolecular sphere (H-Au) for highly sensitive and specific determination of prostate cancer cell-derived exosomes. After integrating the signal amplification effect of the mass cumulative hydrogel and the LSPR effect of AuNPs with high specific aptamer, the SPRi biosensor for exosome detection exhibited a wide linear range from 1.00 × 105 to 1.00 × 107 particles/mL with a limit of detection of 1.00 × 105 particles/mL. Most importantly, with a strong correlation between the SPRi signal and the t-PSA value measured by the clinical chemiluminescence immunoassay, this biosensor displayed excellent practicability for human serum analysis, which exhibits great potential applications in disease diagnosis and bioanalysis.
Surface plasmon resonance biosensor for determination of prostate cancer-derived exosomes
Chen W, Li J, Wei X, Fan Y, Qian H, Li S, Xiang Y, Ding S. (2020) Surface plasmon resonance biosensor using hydrogel-AuNP supramolecular spheres for determination of prostate cancer-derived exosomes. Mikrochim Acta 187(11):590. [abstract]