Exosomes have received significant attention for their role in pathobiological processes and are being explored as a tool for disease diagnosis and management. Consequently, various isolation methods based on different principles have been developed...
Read More »Microfluidic on-demand engineering of exosomes towards cancer immunotherapy
Extracellular Vesicles (EVs), particularly exosomes (30-150 nm), are an emerging delivery system in mediating cellular communications, which have been observed for priming immune responses by presenting parent cell signaling proteins or tumor antigens...
Read More »Isolation of exosomes for the purpose of protein cargo analysis by mass spectrometry
Exosomes are intercellular messengers with a high potential for diagnostic and therapeutic utility. It is believed that exosomes present in body fluids are responsible for providing signals which inhibit immune cells, interfere with antitumor immunity, and thus influence the response ...
Read More »Separation of extracellular nanovesicles and apoptotic bodies from cancer cell culture broth using tunable microfluidic systems
Extracellular vesicles (EVs) are the cell-secreted nano- and micro-sized particles consisted of lipid bilayer containing nucleic acids and proteins for diagnosis and therapeutic applications. The inherent complexity of EVs is a source of heterogeneity in various potential applications of the ...
Read More »Featured Exosome Job – Associate Scientist / Scientist – Cell Biology
Position Overview: Develop scalable cell culture and exosome purification processes to improve production of regenerative medicine products. Key Accountabilities / Responsibilities: Lead efforts in upstream bioprocess development, with particular focus on the efficient expansion of adherent and non-adherent cells (e.g. ...
Read More »Exosome purification from cell culture, and fresh and frozen plasma
In the last decades, several studies demonstrated that the tumor microenvironment is a critical determinant not only of tumor progression and metastasis, but also of resistance to therapy. Exosomes are small membrane vesicles of endocytic origin, which contain mRNAs, DNA ...
Read More »Optimized exosome isolation protocol for cell culture supernatant and human plasma
Extracellular vesicles represent a rich source of novel biomarkers in the diagnosis and prognosis of disease. However, there is currently limited information elucidating the most efficient methods for obtaining high yields of pure exosomes, a subset of extracellular vesicles, from ...
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