Extracellular vesicles (EVs) are phospholipid bilayer membrane-enclosed structures containing RNAs, proteins, lipids, metabolites, and other molecules, secreted by various cells into physiological fluids...
Read More »New insight into isolation, identification techniques and medical applications of exosomes
Exosomes, which are nano-vesicles produced by most cell types, play an irreplaceable role in cell-cell communication. They are extracellular small...
Read More »New Reference Text Published – Extracellular Vesicles – Methods and Protocols
This volume examines established methods and protocols to isolate and characterize extracellular vesicles (EVs) and their composition, among other techniques including purification, imaging, biofluid-specific and cell-specific isolation and downstream genomic and proteomic profiling. The international group of expert scientists who ...
Read More »MicroRNA Profiling of Exosomes
Exosomes are nano-sized membrane-bound vesicles released by a range of different cell types. Exosomes have been shown to specifically package certain membrane and cytosolic proteins and nucleic acids. Furthermore, it has been shown that their contents can be transferred to ...
Read More »A protocol for exosome isolation and characterization
Exosomes are 40-150 nm extracellular vesicles that are released from a multitude of cell types, and perform diverse cellular functions including intercellular communication, antigen presentation, and transfer of tumorigenic proteins, mRNA and miRNA. Exosomes are important regulators of the cellular ...
Read More »High-resolution flow cytometric sorting of different EV subsets
Submicron-sized vesicles released by cells are increasingly recognized for their role in intercellular communication and as biomarkers of disease. Methods for high-throughput, multi-parameter analysis of such extracellular vesicles (EVs) are crucial to further investigate their diversity and function. Researchers at ...
Read More »Recovery of extracellular vesicles from human breast milk is influenced by sample collection and vesicle isolation procedures
Extracellular vesicles (EV) in breast milk carry immune relevant proteins and could play an important role in the instruction of the neonatal immune system. To further analyze these EV and to elucidate their function it is important that native populations ...
Read More »Distinct RNA profiles in subpopulations of extracellular vesicles: apoptotic bodies, microvesicles and exosomes
In recent years, there has been an exponential increase in the number of studies aiming to understand the biology of exosomes, as well as other extracellular vesicles. However, classification of membrane vesicles and the appropriate protocols for their isolation are ...
Read More »The complete exosome workflow solution: from isolation to characterization of RNA cargo
Exosomes are small (30-150 nm) vesicles containing unique RNA and protein cargo, secreted by all cell types in culture. They are also found in abundance in body fluids including blood, saliva, and urine. At the moment, the mechanism of exosome formation, ...
Read More »