Extracellular vesicles (EVs) are small, heterogeneous and difficult to measure. Flow cytometry (FC) is a key technology for the measurement of individual particles, but its application to the analysis of EVs and other submicron particles has presented many challenges and ...
Read More »Improving the scatter sensitivity of a flow cytometer for detection of extracellular vesicles
Extracellular vesicles (EVs) are commonly studied by flow cytometry. Due to their small size and low refractive index, the scatter intensity of most EVs...
Read More »Exosomics implements NanoFCM’s NanoAnalyzer at its Siena facility to provide advanced extracellular vesicles-focused analytical services
Exosomics SpA, the leading extracellular vesicles biotech company, today announced that it has implemented NanoFCM Co., Ltd’s NanoAnalyzer instrument to offer sophisticated contract research and measurement services worldwide. In this strategic partnership, Exosomics becomes NanoFCM’s approved service supplier of nano-flow ...
Read More »High-fidelity detection and sorting of nanoscale vesicles in viral disease and cancer
Biological nanoparticles, including viruses and extracellular vesicles (EVs), are of interest to many fields of medicine as biomarkers and mediators of or treatments for disease. However, exosomes and small viruses fall below the detection limits of...
Read More »Optimisation of imaging flow cytometry for the analysis of single extracellular vesicles
Extracellular vesicles (EVs) mediate targeted cellular interactions in normal and pathophysiological conditions and are increasingly recognised as potential biomarkers, therapeutic agents and drug delivery vehicles. Based on their size and biogenesis, EVs are classified as exosomes, microvesicles and apoptotic bodies. ...
Read More »Centrifugation affects the purity of liquid biopsy-based tumor biomarkers.
Biomarkers in the blood of cancer patients include circulating tumor cells (CTCs), tumor-educated platelets (TEPs), tumor-derived extracellular vesicles (tdEVs), EV-associated miRNA (EV-miRNA), and circulating cell-free DNA (ccfDNA). Because the size and density of biomarkers differ, blood is centrifuged to isolate ...
Read More »Systematic methodological evaluation of a multiplex bead-based flow cytometry assay for detection of extracellular vesicle surface signatures
Extracellular vesicles (EVs) can be harvested from cell culture supernatants and from all body fluids. EVs can be conceptually classified based on their size and biogenesis as exosomes and microvesicles. Nowadays, it is however commonly accepted in...
Read More »Featured Exosome Job – Senior Research Associate-Stem Cell Biology
Celularity is NJ-based biotechnology start-up company striving to become the leader in cellular therapeutics. We are looking for a highly motivated research associate scientist to a group of researchers evaluating novel cell therapies in the field of stem...
Read More »Cushioned-Density Gradient Ultracentrifugation (C-DGUC) of Exosomes
Exosomes represent one class of extracellular vesicles that are thought to be shed by all cell types. Although the exact nature of exosome biogenesis...
Read More »Absolute sizing and label-free identification of extracellular vesicles by flow cytometry
Here, University of Amsterdam researchers present a new method, named flow cytometry scatter ratio (Flow-SR), to relate the ambiguous light scattering signals of flow cytometry to the diameter and refractive index (RI) of single nanoparticles between...
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