Extracellular vesicles (EVs) are released from basically all cells. Over the last decade, small EVs (sEVs; 50-150 nm) have gained enormous attention in diagnostics and therapy. However, methodological limitations coupled to the lack of EV standards leave...
Read More »Optimisation of imaging flow cytometry for the analysis of single extracellular vesicles
Extracellular vesicles (EVs) mediate targeted cellular interactions in normal and pathophysiological conditions and are increasingly recognised as potential biomarkers, therapeutic agents and drug delivery vehicles. Based on their size and biogenesis, EVs are classified as exosomes, microvesicles and apoptotic bodies. ...
Read More »Imaging flow cytometry enhances particle detection sensitivity for extracellular vesicle analysis
Human peripheral blood mononuclear cells were partitioned into five distinct populations using CD45 and side-scatter intensity (SSC). CCD camera acquisition of fluorescent signal conferred increased sensitivity, facilitating the resolution of less common populations such as eosinophils (yellow) and basophils (white), ...
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