Electronic cigarettes (e-cigs) vaping, cigarette smoke, and waterpipe tobacco smoking are associated with various cardiopulmonary diseases. microRNAs are present in higher concentration in...
Read More »Long single-stranded DNAs for electrochemical detection of exosomal microRNAs
Exosomal microRNAs (miRNAs) have been explored as an extremely promising biomarker of liquid biopsy for the diagnosis, treatment and prognosis of diseases such as cancer, in which sensitive and selective detection is significant. Researchers at...
Read More »Identification and validation model for informative liquid biopsy-based microRNA biomarkers
Liquid biopsy-based biomarkers, such as microRNAs, represent valuable tools for patient management, but often do not make it to integration in the clinic...
Read More »Extracellular Vesicles and carried miRNAs in the progression of renal cell carcinoma
The formation and maintenance of renal cell carcinomas (RCC) involve many cell types, such as cancer stem and differentiated cells, endothelial...
Read More »Isolation of exosome-like nanoparticles and analysis of microRNAs derived from coconut water
In this study, researchers at Tongji University identified the presence of microRNAs in coconut water by real-time PCR based on the results of high-throughput small RNA sequencing. In addition, the differences in microRNA content between immature and mature coconut water ...
Read More »Exosomal microRNA as potential diagnostic and prognostic marker in colorectal cancer
Accumulating data have suggested exosome-delivered microRNAs (miRNAs) play critical role in carcinogenesis and cancer progression. However, little is known about the influence of exosomal miR-6803-5p on the development and prognosis of colorectal cancer (CRC). Levels of serum exosomal miR-6803-5p were ...
Read More »Recovery of serum exosomal microRNAs from FTA Elute cards for biomarker discovery and diagnostics
The preservation of nucleic acids from clinical samples is critical to facilitate accurate molecular diagnosis. The use of a paper matrix, Flinders Technology Associates (FTA) Elute cards, to archive DNA and viral RNA is well-documented. However, the feasibility of FTA ...
Read More »Identification of non-invasive miRNAs biomarkers for prostate cancer by deep sequencing analysis of urinary exosomes
Researchers from Oslo University Hospital set out to identify microRNAs in urinary exosomes that are differently expressed in prostate cancer patients and healthy donors. For this purpose, RNA was extracted from urinary exosomes from 20 prostate cancer patients and 9 healthy males ...
Read More »Herpesviruses shape tumour microenvironment through exosomal transfer of viral microRNAs
Metabolic changes within the cell and its niche affect cell fate and are involved in many diseases and disorders including cancer and viral infections. Kaposi’s sarcoma-associated herpesvirus (KSHV) is the etiological agent of Kaposi’s sarcoma (KS). KSHV latently infected cells ...
Read More »A comprehensive method for identification of suitable reference genes in extracellular vesicles
Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is one of the most sensitive, economical and widely used methods for evaluating gene expression. However, the utility of this method continues to be undermined by a number of challenges including normalization using appropriate ...
Read More »