Based on the principle of interaction between matter and light, a new method has been developed to track and observe the Brownian motion of fast-moving nanometer-sized molecules, and measure...
Read More »An assessment of separation methods for extracellular vesicles from human and mouse brain tissues and human CSF
Extracellular vesicle (EV) is a unified terminology of membrane-enclosed vesicular species ubiquitously secreted by almost every cell type and present in all body fluids. They carry a cargo of lipids, metabolites, nucleic acids and proteins for their clearance from cells ...
Read More »Exosomes isolation and identification from equine stem cells
Mesenchymal stem cells are used for different therapeutic approaches, e.g. for osteoarthritis, lesions of the tendon as well as for bone defects...
Read More »Cushioned-Density Gradient Ultracentrifugation (C-DGUC) of Exosomes
Exosomes represent one class of extracellular vesicles that are thought to be shed by all cell types. Although the exact nature of exosome biogenesis...
Read More »High-purity capture and release of circulating exosomes using an exosome-specific dual-patterned immunofiltration (ExoDIF) device
Researchers from the Korea Advanced Institute of Science and Technology present a microfluidic device for the capture and release of circulating exosomes from human blood. The exosome-specific dual-patterned immunofiltration (ExoDIF) device is composed of two distinct immuno-patterned layers, and is ...
Read More »The levels of tumor biomarkers expressed by exosomes may represent a novel, non-invasive clinical tool for the screening and early diagnosis of prostate cancer
Prostate specific antigen (PSA) test is the most common, clinically validated test for the diagnosis of prostate cancer (PCa). While neoplastic lesions of the prostate may cause aberrant levels of PSA in the blood, the quantitation of free or complexed ...
Read More »The Quantification of Human Urinary Exosomes
Wilna Oosthuyzen and Matthew Bailey, from the Centre for Cardiovascular Science, University of Edinburgh, introduce Nanoparticle Tracking Analysis (NPA), a new technique to quantify Human Urinary Exosomes. NPA has proven a faster and more precise method compared to the Western ...
Read More »Analytical challenges of extracellular vesicle detection – A comparison of different techniques
The interest in extracellular vesicles (EVs) has grown exponentially over the last decade. Evolving evidence is demonstrating that these EVs are playing an important role in health and disease. They are involved in intercellular communication and have been shown to ...
Read More »You’ve been doing your exosome isolation all wrong
Exosomes attract enormous research interest because they are carriers of disease markers and a prospective delivery system for therapeutic agents. Differential centrifugation, the prevalent method of exosome isolation, frequently produces dissimilar and improper results because of the faulty practice of ...
Read More »Characterization of exosomes derived from ovarian cancer cells by nanoparticle tracking analysis
Cellular exosomes are involved in many disease processes and have the potential to be used for diagnosis and treatment. In this study, researchers from Peking Union Medical College compared the characteristics of exosomes derived from human ovarian epithelial cells (HOSEPiC) ...
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