Urologic cancers are a heterogeneous group of tumors, some of which have poor prognosis. This is partly due to the unavailability of specific and sensitive diagnostic techniques and monitoring tests, ideally non- or minimally invasive. Hence, liquid biopsies are promising ...
Read More »Identification of non-invasive miRNAs biomarkers for prostate cancer by deep sequencing analysis of urinary exosomes
Researchers from Oslo University Hospital set out to identify microRNAs in urinary exosomes that are differently expressed in prostate cancer patients and healthy donors. For this purpose, RNA was extracted from urinary exosomes from 20 prostate cancer patients and 9 healthy males ...
Read More »Current knowledge on exosome biogenesis and release
Exosomes are nanosized membrane vesicles released by fusion of an organelle of the endocytic pathway, the multivesicular body, with the plasma membrane. This process was discovered more than 30 years ago, and during these years, exosomes have gone from being ...
Read More »Researchers investigate the potential use of lipids in urinary exosomes as prostate cancer biomarkers
Exosomes have recently appeared as a novel source of noninvasive cancer biomarkers, since these nanovesicles contain molecules from cancer cells and can be detected in biofluids. Researchers from the Oslo University Hospital have investigated the potential use of lipids in ...
Read More »Exosomal proteins as prostate cancer biomarkers in urine
Exosomes have recently appeared as a novel source of non-invasive cancer biomarkers since tumor-specific molecules can be found in exosomes isolated from biological fluids. Researchers from Oslo University Hospital have previously analyzed the proteome of urinary exosomes by mass spectrometry, ...
Read More »Magnetic bead-based isolation of exosomes
Exosomes are here defined as extracellular vesicles (EVs) in the approximate size range of 30-100 nm in diameter, and are observed in most body fluids containing typical exosomal markers such as CD9, CD63, and CD81. Potential subpopulations of exosomes can ...
Read More »Exosome-Derived miRNAs and Cellular miRNAs Activate Innate Immunity.
Circulating exosome-containing small RNAs have been demonstrated in vitro to be taken up by recipient cells and to alter gene expression through RNA interference. Here, researchers from the Oslo University Hospital show that exosomes purified from various cancer cell lines ...
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