A team of researchers from Clemson University and Prisma Health–Upstate are working to create a screening process to catch ovarian cancer in the early or pre-cancerous stages. Their goal is to make this screening as simple and easy for women ...
Read More »New Reference Text Published – Extracellular Vesicles – Methods and Protocols
This volume examines established methods and protocols to isolate and characterize extracellular vesicles (EVs) and their composition, among other techniques including purification, imaging, biofluid-specific and cell-specific isolation and downstream genomic and proteomic profiling. The international group of expert scientists who ...
Read More »Charge-based precipitation of extracellular vesicles
Vesicular-mediated communication between cells appears critical in many biological processes. Extracellular vesicles (EVs) released from healthy and diseased cells are involved in a network of exchange of biologically active molecules. Since EVs present in biological fluids carry the signature of ...
Read More »Isolation of Exosome-Like Vesicles from Plants by Ultracentrifugation
Exosomes are nanovesicles of endocytic origin that are about 30-100 nm in diameter, surrounded by a lipid bilayer membrane, and contain proteins, nucleic acids, and other molecules. Mammalian cells- and biological fluids-derived exosomes have become the subject for a wide ...
Read More »Residual matrix from different separation techniques impacts exosome biological activity
Exosomes are gaining a prominent role in research due to their intriguing biology and several therapeutic opportunities. However, their accurate purification from body fluids and detailed physicochemical characterization remain open issues. Researchers from the University of Brescia, Italy isolated exosomes ...
Read More »Optimized exosome isolation protocol for cell culture supernatant and human plasma
Extracellular vesicles represent a rich source of novel biomarkers in the diagnosis and prognosis of disease. However, there is currently limited information elucidating the most efficient methods for obtaining high yields of pure exosomes, a subset of extracellular vesicles, from ...
Read More »Heparin affinity purification of extracellular vesicles
Extracellular vesicles (EVs) are lipid membrane vesicles released by cells. They carry active biomolecules including DNA, RNA, and protein which can be transferred to recipient cells. Isolation and purification of EVs from culture cell media and biofluids is still a ...
Read More »Heparin affinity purification of extracellular vesicles
Extracellular vesicles (EVs) are lipid membrane vesicles released by cells. They carry active biomolecules including DNA, RNA, and protein which can be transferred to recipient cells. Isolation and purification of EVs from culture cell media and biofluids is still a ...
Read More »Methods of Purification of CTL-Derived Exosomes
Exosomes are membrane nanovesicles (approximately <120 nm in size) released by most, if not all, living cells and in particular by leukocytes. They originate within the endocytic compartment by invagination of the endosome membrane. Therefore, they have a different biogenesis ...
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