Extracellular vesicles (EVs) are membranous vesicles secreted by both prokaryotic and eukaryotic cells and play a vital role in intercellular communication. EVs are classified into several subtypes based on their origin, physical characteristics, and biomolecular...
Read More »Improved isolation strategies to increase the yield and purity of human urinary exosomes for biomarker discovery
Circulating miRNAs are detected in extracellular space and body fluids such as urine. Circulating RNAs can be packaged in secreted urinary extracellular vesicles (uEVs) and thus protected from...
Read More »Isolation of exosomes for the purpose of protein cargo analysis by mass spectrometry
Exosomes are intercellular messengers with a high potential for diagnostic and therapeutic utility. It is believed that exosomes present in body fluids are responsible for providing signals which inhibit immune cells, interfere with antitumor immunity, and thus influence the response ...
Read More »Isolation of milk extracellular vesicles by size-exclusion chromatography
Studies have suggested that nanoscale extracellular vesicles (EV) in human and bovine milk carry immune modulatory properties which could provide beneficial health effects to infants. In order to assess the possible health effects of milk EV, it is essential to ...
Read More »Nanosized UCMSC-derived extracellular vesicles exclusively inhibit the inflammatory response of stimulated T cells
Undesired immune responses have drastically hampered outcomes after allogeneic organ transplantation and cell therapy, and also lead to inflammatory diseases and autoimmunity. Umbilical cord mesenchymal stem cells (UCMSCs) have powerful regenerative and immunomodulatory potential, and their secreted extracellular vesicles (EVs) ...
Read More »Izon Science releases exosome isolation column
Izon Science announces the release and immediate availability of its new analytical-scale exosome isolation column, the qEVsingle. Based on Size Exclusion Chromatography, the qEVsingle is designed to handle 100μL samples of biological fluids (blood plasma or serum, CSF, saliva, urine, ...
Read More »Isolation of Exosomes from Blood Plasma – Qualitative and Quantitative Comparison of Ultracentrifugation and Size Exclusion Chromatography Methods
Exosomes are emerging targets for biomedical research. However, suitable methods for the isolation of blood plasma-derived exosomes without impurities have not yet been described. Therefore, researchers from Semmelweis University, Hungary investigated the efficiency and purity of exosomes isolated with potentially ...
Read More »Exosomes – isolation methods and specific markers
from Labome by Konstantin Yakimchuk Various methods for isolation of exosomes from biological fluids have been developed. They include centrifugation, chromatography, filtration, polymer-based precipitation and immunological separation. Recent technical improvements in these methods have made the isolation process faster and ...
Read More »Isolation of biologically-active exosomes from human plasma
Effects of exosomes present in human plasma on immune cells have not been examined in detail. Immunological studies with plasma-derived exosomes require their isolation by procedures involving ultracentrifugation. These procedures were largely developed using supernatants of cultured cells. To test ...
Read More »Towards traceable size determination of extracellular vesicles
Extracellular vesicles (EVs) have clinical importance due to their roles in a wide range of biological processes. The detection and characterization of EVs are challenging because of their small size, low refractive index, and heterogeneity. In this manuscript, the size ...
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