Biological nanoparticles, including viruses and extracellular vesicles (EVs), are of interest to many fields of medicine as biomarkers and mediators of or treatments for disease. However, exosomes and small viruses fall below the detection limits of...
Read More »Obstacles and opportunities in the functional analysis of extracellular vesicle RNA
The release of RNA-containing extracellular vesicles (EV) into the extracellular milieu has been demonstrated in a multitude of different in vitro cell systems and in a variety of body fluids. RNA-containing EV are in the limelight for their capacity to ...
Read More »MVP-mediated selective sorting of tumour suppressor miRNA into exosomes promotes tumour progression
Exosomes are emerging mediators of intercellular communication; whether the release of exosomes has an effect on the exosome donor cells in addition to the recipient cells has not been investigated to any extent. Here, researchers from the University of Louisville ...
Read More »Small RNA analysis in exosomes provides insights into localized RNA processing, degradation and sorting
Exosomes are small vesicles that mediate cell-cell communication. They contain proteins, lipids and RNA, and evidence is accumulating that these molecules are specifically sorted for release via exosomes. Researchers from UMC Utrecht recently showed that endothelial-cell-produced exosomes promote angiogenesis in ...
Read More »Exosome and Exosomal MicroRNA – Trafficking, Sorting, and Function
Exosomes are 40-100 nm nano-sized vesicles that are released from many cell types into the extracellular space. Such vesicles are widely distributed in various body fluids. Recently, mRNAs and microRNAs (miRNAs) have been identified in exosomes, which can be taken ...
Read More »High-resolution flow cytometric sorting of different EV subsets
Submicron-sized vesicles released by cells are increasingly recognized for their role in intercellular communication and as biomarkers of disease. Methods for high-throughput, multi-parameter analysis of such extracellular vesicles (EVs) are crucial to further investigate their diversity and function. Researchers at ...
Read More »Sorting it out: Regulation of exosome loading
Extracellular vesicles (EVs), a term that includes both exosomes of endocytic origin and vesicles derived from plasma membranes, are continuously secreted by cells to the extracellular environment, and represent a novel vehicle for cell-cell communication. Exosomes contain specific repertoires of ...
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