Biomarkers in the blood of cancer patients include circulating tumor cells (CTCs), tumor-educated platelets (TEPs), tumor-derived extracellular vesicles (tdEVs), EV-associated miRNA (EV-miRNA), and circulating cell-free DNA (ccfDNA). Because the size and density of biomarkers differ, blood is centrifuged to isolate ...
Read More »Absolute sizing and label-free identification of extracellular vesicles by flow cytometry
Here, University of Amsterdam researchers present a new method, named flow cytometry scatter ratio (Flow-SR), to relate the ambiguous light scattering signals of flow cytometry to the diameter and refractive index (RI) of single nanoparticles between...
Read More »Methodological Guidelines to Study Extracellular Vesicles
All body fluids contain cell-derived membrane-enclosed vesicles. Such vesicles are shed by prokaryotes and eukaryotic cells and contain messages to the environment. Cell-derived vesicles are thought to contribute to homeostasis, disease development, and progression, may provide novel biomarkers, and may ...
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