Extracellular vesicles (EVs) are small lipid-enclosed particles that can carry various types of cargo, including proteins, nucleic acids and metabolites...
Read More »Pitfalls associated with lipophilic fluorophore staining of extracellular vesicles for uptake studies
Post-staining of extracellular vesicles (EVs) with lipid-anchored fluorophores (LAFs) such as PKH67 is a widely used strategy for studying EVs but it is associated with several pitfalls. The pitfalls discussed in this commentary are related to LAF labelling of non-EV ...
Read More »Discovery that ‘size matters’ in cell-to-cell communication could unlock new methods for disease diagnosis and treatment
Size really does matter when it comes to the mechanisms that cells use to communicate with each other, according to pioneering new nanobiotechnology research which has important implications for the diagnosis and treatment of disease. An international team of scientists ...
Read More »Extracellular Vesicle Docking at the Cellular Port
Extracellular vesicles (EVs), lipid bilayer-enclosed structures that contain a variety of biological molecules shed by cells, are increasingly becoming appreciated as a major form of cell-to-cell communication. Indeed, EVs have been shown to play important roles in several physiological processes, ...
Read More »Effective extracellular vesicle-based intracellular delivery of an artificially encapsulated protein
Extracellular vesicles (EVs, exosomes) are approximately 30- to 200-nm-long vesicles that have received increased attention due to their role in cell-to-cell communication. Although EVs are highly anticipated to be a next-generation intracellular delivery tool because of their pharmaceutical advantages, including ...
Read More »Human vascular endothelial cells transport foreign exosomes from cow’s milk and deliver the microRNA cargo to peripheral tissues
Encapsulation of microRNAs in exosomes confers protection against degradation and a vehicle for shuttling of microRNAs between cells and tissues, and cellular uptake by endocytosis. Exosomes can be found in foods including milk. Humans absorb cow’s milk exosomes and deliver ...
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