The 3rd International Symposium on Microgenomics 2023 – June 28 (Workshop), June 29-30 (Symposium), 2023
This technical workshop is coordinated by Michael Pfaffl from the Life Science Center Weihenstephan Technical University of Munich Munich, Germany.
Extracellular Vesicle (EV) are important for the cellular communication in any organism and will be used in molecular diagnostics.
The EV workshop will give you a first introduction into EVs and explain the MISEV guidelines.
Furthermore, various EV isolation methods and EV characterisation methods (e.g. NTA) will be presented.
A focus will be on EV protein and EV RNA biomarkers, how to find and quantify these, e.g. via NGS, PCR and bioinformatical tools.
- Introduction & MISEV Guidelines – Michael Pfaffl, TUM, School of Life Sciences, Freising, Germany
- Extracellular Vesicles – How to find the needle in the haystack – Christian Preusser, EV Core Facility, Medical Faculty, University Marburg, Germany
- Convenient and Robust Isolation of EV RNA and intact EV – Martin Schulmpberger, QIAGEN R&D, Hilden, Germany
- Next Generation of NTA – Fluorescence Colocalisation Measurements – Christina Klasen, Particle Matrix R&D, Inning am Ammersee, Germany
- Coffee Break
- EV Biomarker Discovery – Comparison of EV Isolation Methods, small-RNA Seq and RT qPCR validation – Michael Pfaffl, TUM, School of Life Sciences, Freising, Germany
- Digital PCR-Based Detection and Characterization of Extracellular Vesicle Biomarkers – Afif Abdel Nour & Ellen Bruske, Qiagen R&D, Hilden, Germany
- caRNAge — comprehensive small RNA Seq data analysis – Benedikt Kirchner, TUM, School of Life Sciences, Freising, Germany
- Final Q&A and Discussion