WO2020133912 – Method for simultaneously detecting exosome membrane protein and mRNA

Publication Number WO/2020/133912
Publication Date 02.07.2020
International Application No. PCT/CN2019/089478
International Filing Date 31.05.2019

Provided in the present invention is a method for simultaneously detecting exosome membrane protein and mRNA. The present method can, in the same isolated and purified exosome sample, perform simultaneous detection using fluorescent antibody tagging of the exosome membrane protein and molecular beacon tagging of target gene mRNA included in the exosome, the molecular beacon tagging of the exosome specifically being detected using an exosome in situ capture well plate or a chip, each well of the exosome in situ capture well plate or the chip having therein a fluorescein tagging molecular beacon, the molecular beacon being a specific DNA probe detecting target gene mRNA. The present invention uses exosome in situ capture well plate and chip technology, detecting biomarker gene mRNA included in an exosome in a biological sample.