Methodologies to isolate and purify clinical grade extracellular vesicles for medical applications

The use of extracellular vesicles (EV) in nano drug delivery has been demonstrated in many previous studies. In this study, Researchers from the Stanford University School of Medicine discuss the sources of extracellular vesicles, including plant, salivary and urinary sources which are easily available but less sought after compared with blood and tissue. Extensive research in the past decade has established that the breadth of EV applications is wide. However, the efforts on standardizing the isolation and purification methods have not brought us to a point that can match the potential of extracellular vesicles for clinical use. The standardization can open doors for many researchers and clinicians alike to experiment with the proposed clinical uses with lesser concerns regarding untraceable side effects. It can make it easier to identify the mechanism of therapeutic benefits and to track the mechanism of any unforeseen effects observed.

Schematic representation of different methods for extracellular isolation and purification.

Akbar A, Malekian F, Baghban N, Kodam SP, Ullah M. (2022) Methodologies to Isolate and Purify Clinical Grade Extracellular Vesicles for Medical Applications. Cells 11(2), 186. [article]

Leave a Reply

Your email address will not be published. Required fields are marked *